J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (1): 107-110.

• Research Articles • Previous Articles    

A Preliminary Study on Genetic Variation and Relationships of Pinus yunnanensis and Its Closely Related Species

YU Hong, GE Song, HUANG Rui-Fu, JIANG Han-Qiao   


The authors investigated the genetic diversity of 29 natural populations representing Pinus yunnanensis Franch. and its two close relatives, P. densata Mast. and P. kesiya Royle ex Gordn. var. langbianensis (A Chey.) Gaussen. Horizontal starch gel electrophoresis was performed for macrogametophytes collected from populations in Yunnan, Sichuan and Guangxi. Allozyme data for 33 loci of 14 enzymes demonstrated high levels of genetic variation at both population and species levels in comparison with other conifers, with the mean values for populations being P=0.694, A=2.0 and He=0.145 for P. yunnanensis; P=0.714, A=2.0 and He=0.174 for P. densata; and P=0.758, A=2.1 and He=0.184 for P. kesiya var. langbianensis. Based on Wright's F-statistics, the fixation index of P. yunnanensis, P. densata and P. kesiya var.langbianensis were 0.101, 0.054 and 0.143, respectively, indicating that the populations were largely under random mating. Based on Nei's genetic distance, the genetic differentiation was not obvious among the three species (i.e. the genetic distance was less than 0.075). Because of the wider distribution of P. yunnanensis with greater variety of habitats, it was shown that the genetic differentiation among the P. yunnanensis populations was larger than that of the populations of the other two species. According to morphological, geographic and allozymic evidences, the authors suggested that the three species be better treated as varieties under a single species. In addition, the extensive gene flow among the three pine species resulted in great genetic diversity and evolutionary potential. Also, high level of genetic variation of P. yunnanensis provides important basis for its genetic improvement and breeding in future.

虞泓1,2  葛颂1* 黄瑞复2  姜汉侨2

(1. 中国科学院植物研究所系统与进化植物学开放研究实验室,北京10009;2. 云南大学生命科学与化学学院,昆明650091)
关键词: 云南松;高山松;思茅松;等位酶;遗传多样性

Key words: Pinus yunnanensis, Pinus densata, Pinus kesiya var. langbianensis, allozymes, genetic diversity

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