J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (3): 327-330.

• Research Articles • Previous Articles    

Confocal Fluorescence Microscopic Observation on the Transcellular Distribution of Actin Filaments in the Epidermis of Garlic Clove Sheath

WANG Dong-Mei, WANG Xue-Chen, ZHANG Wei-Cheng   


By means of paraformaldehyde fixation, Triton X-100 extraction and TRITC-phalloidin staining, the presence and distribution patterns of F-actin in the outer epidermal cells of the garlic (Allium sativum L.) sheath were studied with fluorescence probe technique and confocal laser scanning microscopy. There were a lot of actin filaments (AFs) impenetrate the cell wall, but the AFs with red fluorescence were absent when the cells were treated with cytochalasin D before fixation; the same result was obtained when the cells were treated with unlabeled phalloidin. These results indicate the presence of F-actin in the intercellular channels and that it is related to the plasmodesmata and intercellular trafficking of macromolecules.

王冬梅1,2 王学臣2* 张伟成3

(1. 河北农业大学农学院,保定071001;2.  中国农业大学生物学院,北京100094;#$ 中国科学院上海植物生理研究所,上海200032)
关键词: 胞间连丝;F-肌动蛋白;异硫氰四甲基罗丹明*鬼笔环肽;细胞松弛素-D;激光共聚焦扫描显微镜检术;蒜

Key words: plasmodesmata, F-actin, TRITC-phalloidin, cytochalasin D, confocal laser scanning microscopy (CLSM), Allium sativum

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