J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (5): 480-484.

• Research Articles • Previous Articles     Next Articles

Genetic Transformation of Watercress with a Gene Encoding for Betaine-aldehyde Dehydrogenase (BADH)

LI Yin-Xin, CHANG Feng-Qi, DU Li-Qun, GUO Bei-Hai, LI Hong-Jie, ZHANG Jin-Song, CHEN Shou-Yi, ZHU Zhi-Qing   

Abstract:

Betaine-aldehyde dehydrogenase (BADH) gene was transferred into watercress (Nasturtium officinale R. Br.) by Agrobacterium-mediated transformation. PCR and Southern blotting analysis showed that the BADH gene was integrated into genome of 46 watercress plants. Among the 6 transgenic plants being examined, 5 showed higher BADH activity than the control. Northern blotting analysis of the 5 plants showed that 4 with higher BADH activity had BADH transcript expression. Relative electronic conductivity demonstrated that less membrane damage of transgenic plants. Some transgenic plants grew normally on the medium with 0.5% NaCl while the control plants had very poor roots and slow grew in the same medium. The growth of some transgenic plants were superior to control obviously on the medium with 0.8% NaCl, although they grew slower than on the medium with 0.5% NaCl. And the leaves of control turned yellow after about 20 days to 1 month of cultured and eventually died.

转甜菜碱醛脱氢酶基因豆瓣菜的耐盐性
李银心 常凤启1,2 杜立群1  郭北海3  李洪杰3,4 张劲松4 陈受宜4  朱至清1*

(1. 中国科学院植物研究所,北京100093;2. 北京师范大学生命科学学院,北京100875;
3.  河北省农林科学院粮油作物研究所,石家庄050031;4. 中国科学院遗传研究所,北京100101)

摘要:将山菠菜甜苹碱醛脱氢酶(BADH)基因经根癌土壤杆菌(Agrobacterium tumefaciens (Smith et Townsend)Conn)AGL1介导转入豆瓣菜(Nasturtium nofficinale R,Br.)PCR、Southerj bloting检测呈阳性的再生植株有46株,对6株再生植株的BADH活性和Northern bloting检测发现,有5株BADH酶活性明显高于对照;膜的相对电导率测定结果说明,在盐胁迫下转基因豆瓣菜的膜结构所受损伤小于对照。转基因植株能够在0.5%NaC1正常生长,而对照在相同的培养基上生根困难且生长缓慢。在0.8%NaC1 的培养基上,部分转基因植株虽然生长减慢,但生长状况明显优于对照,且对照植株在相同培养基上培养20d 到1个月左右后,叶片变黄并最终死亡。
关键词: 甜菜碱醛脱氢酶基因;转基因植株;耐盐性;豆瓣菜

Key words: Betaine-aldehyde dehydrogenase gene, transgenic plant, salt-tolerance, watercress

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