J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (7): 728-731.

• Research Articles • Previous Articles     Next Articles

Inheritance and Sequence Homology Analysis of the Maize DNA Introgressed into the Wheat Doubled Haploid Plant Through Wheat×Maize Cross

CHEN Chun-Xian, SUN Jing-San, ZHU Li-Huang   

Abstract:

A maize (Zea mays L.) genome-specific repeated DNA sequence (clone MR64) has been transferred into one DH line of wheat through wheat (Triticum persicum Vav. ex Zhuk.) and maize cross. In the present study by RFLP analysis the authors proved that this DNA sequence could stably transmit into DH3 plants, the next generation derived from DH2 self-crossing. A similarity search in all DNA databases using BLASTN program showed that the DNA sequence of MR64 had as high as 93% identity to PREM-2 and 79% to Opie-2 in nucleotides. Both PREM-2 and Opie-2 are known as retrotransposons in maize genome, suggesting that MR64 likely is the partial sequence of a maize retrotransposon. Therefore, the results indicate that some retrotransposon might involve the DNA introgression from maize to wheat genome through wide fertilization. Stable inheritance of this maize genome-specific retrotransposon-like DNA in the wheat genome opens up the possibility of using retrotransposon as a new tool for gene tagging, function analysis, and insertional mutagenesis in wheat genome.

导入小麦加倍单倍体植株的玉米DNA在后代中的遗传及序列同源性分析
陈纯贤1,2 孙敬三2  朱立煌1*

(1. 中国科学院遗传研究所,北京100101;2. 中国科学院植物研究所,北京100093)

摘要:作者曾报道了一个玉米(Zea mays L.)基因组特异的重复序列DNA(克隆MR64)通过小麦(Triticum persicum Vav.ex Zhuk.)与玉米杂交导入一个小麦加倍单倍体(DH)植株中。利用分析主宰该重复DNA序列可以稳定地传递到小麦DH3代植株。通过Intermet在DNA数据库中进行序列相似性搜寻和同源性比较,结果显示,MR64的DNA序列和玉米的最近报道的两个逆转座子PREM-2和QPIE-2分别有高达93%和79%的序列同一性。因此,该玉米DNA片段很可能是一种逆转座子的一部分,这意味着有可能利用玉米的某些基因组特异的逆转座子的序列在小麦基因组中进行基因标签、功能分析和插入突变的研究。
关键词: 小麦;玉米;远缘杂交;遗传传递;逆转座

Key words: wheat, maize, wide crosses, genetic transmission, retrotransposon

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