J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (2): 210-212.

• Research Articles • Previous Articles     Next Articles

Cloning and Analysis of a cDNA Encoding psbL and psbJ Gene in Rice Chloroplast Genome

GU Ke-Yu, LUO Lin-Guang, SU Chang-Chao, ZHAI Hu-Qu   

Abstract:

A 505 bp cDNA was cloned from the leaves of rice ( Oryza sativa L.) Shanyou 63 combination. DNA sequence analysis showed that it is a part of rice chloroplast genome. Its homology comparison with those known in GenBank found that it encodes 38 amino acid peptide deduced from psbL gene and 40 amino acid peptide deduced from psbJ gene in rice chloroplast PSⅡ. Northern hybridization showed that the cDNA was differentially displayed in hybrid F1 and its parental lines.

水稻叶绿体基因组中一个编码 psbL  和psbJ 基因cDNA 的克隆与分析
顾克余*  罗林广 苏昌潮 翟虎渠
(南京农业大学作物遗传与特异种质创新教育部重点实验室,南京210095)
关键词: 水稻;psbL psbJ;cDNA 克隆;基因表达;差异显示

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