J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (3): 232-237.

• Research Articles • Previous Articles     Next Articles

Agronomic Trait and Protein Component of F2 Hybrid Originated from Intergeneric Somatic Hybridization Between Triticum aestivumand Agropyron elongatum

XIANG Feng-Ning, FENG Bao-Min, XIA Guang-Min, CHEN Hui-Min   


Protoplasts derived from common wheat ( Triticum aestivum L. cv. Jinan 177) were fused with UV-treated protoplasts of Agropyron elongatum (Host) Nevski by PEG method, and fertile asymmetric somatic hybrid plants resembling wheat morphology were obtained. The F2 hybrid plants could be divided into 3 types according to their morphology. Type Ⅰ hybrids had high and loosely standing stalks with big spikes and grains. TypeⅡ hybrids were dwarf and compact in shape with high tillering ability and smaller spikes. Type Ⅲ hybrids were similar to typeⅠas a whole but had more compact and erect spikes. All the F2 hybrid lines were superior to wheat in seed protein content, although some difference existed between themselves. Protein analysis of immature embryos and flag leaves from hybrids by two-dimensional electrophoresis showed that they possessed characteristic proteins of both parents and some new proteins. There existed also some different kinds of proteins in different lines.

小麦与高冰草体细胞杂种F2 性状和蛋白质组分
向凤宁 保民夏 夏光敏* 陈惠民

摘要: 普通小麦(Triticum aestvum L.)“济南177”原生质体和经紫外线照射的高冰草(Agropyton elongatum (Host)Nevski)原生质体(供体)用PEG法诱导融合,形成外形偏像小麦的不对称体细胞杂种植株及后代,F2代株系可分为3种类型:1.株型松散、大穗大粒;2.株型直立紧凑、分蘖多、中穗型;3.大穗大粒直立型。蛋白质含量明显优于亲本小麦,株系之间也存在显著差异;采用微IEF/SDS-PAGE双向电泳技术分析杂种F2代植株旗叶及幼胚蛋白质组分可见,杂种不仅含有双亲的蛋白质组,而且还产生了特异的新蛋白;不同杂种株系间蛋白质组分差异明显。

关键词: F2代体细胞杂种后代;小麦;高冰草;双向电泳;粗蛋白含量;蛋白质组分

Key words: F2 somatic hybrid plant, Triticum aestivum , Agropyron elongatum , two-dimensional electrophoresis, crude protein, protein component

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