J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (3): 323-325.

• Research Articles • Previous Articles     Next Articles

Phylogenetic Relationship ofPopulusSections by ITS Sequence Analysis

SHI Quan-Liang, ZHUGE Qiang, HUANG Min-Ren, WANG Ming-Xiu   


ITS sequences of 15 representative species of five sections in the genus Populus L. were determined. By using direct sequencing of PCR product, it was found that the fragments of internal transcribed spacers (ITS) are about 594 bp in length. The length of ITS-1 and ITS-2 is about 220 bp and 210 bp, respectively, while that of 5.8s is 164 bp. Its G+C content is about 69.0%. The number of phylogenetically informative loci is higher in ITS-2 than in ITS-1. Transversion and transition are two main factors that drive the ITS evolution, and more insertions and deletions occurred in ITS-2. Taking Salix matsudana Koidz. and Salix suchowensis Cheng as outgroups, phylogenetic analysis of ITS sequences using PAUP 4.0 software indicated that Populus is monophyletic group and can be divided into two main clades. One is the section Leuce , and the other is the remaining sections.

史全良 诸葛强 黄敏仁*王明庥
关键词: 杨树;内转录间隔区(ITS)序列;系统发育

Key words: Populus , internal transcribed spacers (ITS) sequence, phylogeny

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q