J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (6): 650-652.

• Research Articles • Previous Articles     Next Articles

Isolation of Plasma and Thylakoid Membranes from the Heterocystous Cyanobacterium Anabaena sp. PCC 7120

LI Bin, XU Dong-Yi, ZHAO Jin-Dong   

Abstract:

具异型胞蓝细菌 Anabaena sp.PCC 7120质膜和类囊体膜的分离纯化
李斌 徐冬一 赵进东*

(北京大学生命科学学院,北京100871)

摘要:利用水溶性多聚体双相法分离蓝细菌Anabaena sp .PCC 712 0质膜和类囊体膜两种膜系统。吸收光谱分析表明 ,质膜相和类囊体膜相的主要色素分别为类胡萝卜素和叶绿素。SDS-凝胶电泳显示这两种膜系统蛋白组成有很大差别。这种分离方法容易操作 ,对研究蓝细菌的膜蛋白和膜脂非常有用。

关键词: 蓝细菌;质膜和类囊体膜;分离和纯化;双相法

Key words: Cyanobacterium, plasma and thylakoid membranes, isolation and purification, two-phase partition

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