The treatment of wheat ( Triticum aestivum L.) seedlings with CaCl2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca2+ chelator EGTA decreased the expression of the hsp26 gene. The expression of the wheat calmodulin gene CaM1-2 is rapidly up-regulated in wheat seedlings by heat shock at 37 ℃. The heat-induced up-regulation of CaM1-2 occurred earlier than that of the hsp26 gene did. Calmodulin antagonist N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamid (W7) inhibited the expression of the hsp26 gene in wheat seedlings at 37 ℃ for 1 h. These results implied that the calcium-calmodulin is probably involved in the signal transduction of the hsp26 gene expression induced by heat shock.
刘宏涛1,2 赵和1 李冰1,2 孙大业2 周人纲1*