J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (10): 1173-1181.

• Research Articles • Previous Articles     Next Articles

Genetic Relationships Among Soluble Carbohydrates, Anthocyanins and Growth Characteristics in Leymus (Gramineae) Detected with Molecular Markers 

HU Zan Min*, Steven R. LARSON, Thomas A. JONES, Richard R   

  • Published:2002-10-21


Low temperature soluble carbohydrate accumulations are commonly associated with anthocyanin coloration, attenuated growth and cold adaptation of cool season grasses. The vrn-1 gene has potent effects on vernalization requirement, growth, and soluble carbohydrate accumulations of the winter annual Triticeae species. Two hundred and four unmapped AFLP markers and genome specific DNA markers genetically linked to the vrn-1 gene were used to detect QTL controlling soluble carbohydrate accumulations, anthocyanin coloration and growth characteristics in a segregating population derived from open pollinated Leymus cinereus×L. triticoides hybrids. These perennial Triticeae grasses are distinguished by adaptation and growth habit. As expected, positive trait correlations and pleiotropic gene effects were detected for soluble carbohydrate accumulations and anthocyanin coloration. Likewise, positive trait correlations and pleiotropic gene effects were detected for tillering, leaf development, leaf growth, regrowth and rhizome spread. However, soluble carbohydrate accumulations were not associated with attenuated growth. In fact, several DNA marker alleles, including one near vrn-Xm1, had positive effects on soluble leaf carbohydrate concentrations and low temperature growth. The corresponding DNA marker near vrn-Ns1 had more specific effects on tillering. We speculate that vrn-1 exerts quantitative effects on low temperature soluble leaf carbohydrate accumulations and growth habit of the perennial Leymus. However, a number of other DNA markers displayed highly significant effects on soluble carbohydrate accumulations and various growth characteristics. Findings indicate that anthocyanin coloration may be a useful phenotypic marker for soluble carbohydrate accumulation. Although variation for soluble carbohydrates was not associated with attenuated growth in this population, this trait was under genetic control.

胡赞民1,2* Steven R.LARSON 2* Thomas A.JONRS2 Richard R-C.WANC 2

(1.中国科学院遗传与发育生物学研究所,北京!100101;2. U.S.Department of Agriculture, Agriculture Research Service,Research Laboratory Utah State Unixersity,Logan,UT 84322-6300,USA)

摘要: 在低温条件下,冷季草的可溶性碳水化合物通常与紫色素的积累、生长的缓慢性及对寒冷的适应性具有相关性. Vrn-1基因对一年生冬性禾本科物种的春化、生长和可溶性碳水化合物的积累有重要作用.本研究以Leymus cinereus×L. triticoides 的杂种F 1开放授粉获得的F 2群体为材料,用204个未定位的AFLP分子标记和几个基因组特定的与 vrn-1相连锁的DNA标记检测了控制可溶性碳水化合物的积累、紫色素的积累和生长特性等几个数量性状的QTL.根据生长特性和适应性可将 Leymus cinereusL. triticoides区分开来.研究表明可溶性碳水化合物与紫色素的积累呈正相关,而且有关基因对这两种性状具有多效性.与之相类似,分蘖、叶发育、叶生长、草被剪后的再生长和地下茎的蔓延性这些性状之间也呈正相关,控制这些性状的基因具有多效性.但是可溶性碳水化合物的积累与生长的缓慢性无相关性.有几个分子标记包括与vrn-Xm1 邻近的一个分子标记对叶可溶性碳水化合物的浓度和低温生长具有正效应.而与vrn-Ns1邻近的一个DNA标记对分蘖具有更加特别的效应.我们推测vrn-1对多年生赖草低温下叶可溶性碳水化合物的积累及生长习性具有数量效应.发现几个DNA标记对可溶性碳水化合物的积累及多个生长特性有较强的作用.研究结果暗示在赖草中紫色素的积累可作为可溶性碳水化合物积累的形态标记.

关键词: 赖草属;分子标记;遗传相关性

通讯作者。E-mail: zmhu @ genetics.ac.cn.

Key words: Leymus, molecular markers, genetic relationship

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