J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (2): 177-180.

• Research Articles • Previous Articles     Next Articles

Molecular Tagging of a New Resistance Gene to Maize Dwarf Mosaic Virus Using Microsatellite Markers

WU Jian-Yu, TANG Ji-Hua, XIA Zong-Liang and CHEN Wei-Cheng   

Abstract:

With joint analysis based on the parents, F1, F2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1 (t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077 mdm1(t) and mdm1(t) bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.

一个新的抗玉米矮花叶病基因位点的微卫星标记

吴建宇  汤继华  夏宗良  陈伟程

(河南农业大学农学院,郑州 450002)

摘要:通过混合遗传模型P1P2B1B2F1F2 6世代联合分析发现, 玉米(Zea mays L.)自交系黄早四对玉米矮花叶病B株系的抗性是由一对主基因和多基因共同控制,从而鉴别出一对主效基因的存在;利用位于第六染色体上的27对微卫星标记,对黄早四×Mo17 F2群体进一步分析,筛选出两个与主效抗病基因(mdm1(t))紧密连锁的微卫星标记phi077 bnlg391,它们在分子图谱上的顺序为phi077-mdm1(t)-bnlg391,两个区间的遗传距离分别是4.74 centiMorgan (cM)6.72 cM.

关键词 玉米 玉米矮花叶病 抗病基因 微卫星 分子标记

 

Key words: maize, maize dwarf mosaic virus, resistance gene, microsatellite, molecular tagging

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