Abstract:

Proteomics is one of the most active research fields in the post genomic era. Here we briefly introduce the scientific background of the origination of proteomics and its content, research method. The new developments of proteomics at the levels of individual plants, tissues, organs and organells, as well as its applications in the area of plant genetic diversity, mutant characterization, and plant physiology, etc are reviewed. At last, the challenge and prospect of proteomics are discussed.

后基因组时代的植物蛋白质组学
郭奕明 沈世华 荆玉祥*  匡廷云
（中国科学院植物研究所，北京100093）

摘要： 蛋白质组学是后基因组时代功能基因组学研究的新兴学科和热点领域。简要介绍了蛋白质组学产生的科学背景、研究内容和研究方法。重点综述了植物个体水平、组织、器官和亚细胞水平蛋白组研究，植物蛋白质组学在植物遗传多样性、遗传突变体、植物的逆境生理等方面的研究进展。最后展望了今后的发展前景。
关键词： 基因组；后基因组；蛋白质组；植物蛋白质组学；基质辅助激光解吸] 电离飞行时间质谱法（MALDI-TOF/MS); ；二维聚丙烯酰胺凝胶电泳(2D-PAGE)

通讯作者。E-mauk: <yxjing @ ns.ibcas.ac.cn>.

Key words: genome, post genome, proteome, plant proteomics, matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS), two dimensional polyacrylamide gel electrophoresis (2D PAG