J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (8): 936-940.

• Research Articles • Previous Articles     Next Articles

Isolation and Analysis of Rubber Hevein Gene and Its Promoter Sequence

DENG Xiao-Dong, FEI Xiao-Wen, HUANG Jun-Sheng and ZHENG Xue-Qin   

Abstract:

Hevein, a lectin like protein, is a major factor of lutoids in the latex of rubber trees (Hevea brasiliensis Muell. Arg.). This factor is involved in coagulation of the latex and has the ability to bind chitin. The hevein gene with a length of 680 bp was cloned by the method of RT PCR. Its promoter region with 1 306 bp of this gene was also isolated by genome walking, and its sequence included the typical TATA and CAAT boxes as well as the homologous sequence of abscisic acid (ABA)response elements. Expression of the hevein gene in the latex and leaves was detected by Northern blot. After treatment of the trees with ethylene and ABA, the results showed that the hevein gene was expressed principally in latex, and the expression could be induced by ethylene and ABA.

橡胶树凝集因子hevein 基因及其启动子序列的分离与分析
邓晓东1费小雯2黄俊生1 郑学勤1

(1. 中国热带农业科学院热带作物生物技术国家重点实验室,海口571101;2. 中山大学生命科学学院生物工程中心,广州510275)

摘要:橡胶树 (Heveabrasiliensis Muell._Arg .)凝集因子hevein是引起橡胶粒子凝集的主要因素 ,它是胶乳中黄色体内主要的蛋白质 ,具有几丁结合的功能。通过PCR技术扩增并克隆了橡胶树hevein基因共 6 80bp的序列。继而通过步移法分离启动子区域 130 6bp的序列 ,序列含典型的TATA盒和CAAT盒以及ABA效应元件的同源序列。为证实该基因在乳管中特异表达 ,利用Northernblot分析hevein基因在胶乳和叶片中的表达 ,同时 ,分析乙烯和ABA处理后hevein基因的表达。结果表明 ,hevein基因主要在胶乳中表达 ,乙烯和ABA对基因的表达有诱导作用。

关键词: 橡胶树;凝集因子hevein;启动子;Northem blot.

Key words: rubber tree, hevein, promoter, Northern blot

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