Biotransformation of Paclitaxel (Taxol) by the Cell Suspension Cultures of Rauwolfia serpentina

Abstract

Abstract:

The biotransformation of paclitaxel (TaxolⓇ) by the cell suspension cultures of Rauwolfia serpentina (L.) Benth. et Kurz. were investigated. Three Paclitaxel-based intracellular metabolites were detected from the cell filter cake and were, by high field ¹H-NMR and MS data, identified as 10-deacetyltaxol, baccatin Ⅲ, and 10-deacetylbaccatin Ⅲ. No glucosidated or hydroxylated derivatives were checked out in this incubation experiment.

Key words: biotransformation, cell culture, Paclitaxel, taxanes, Rauwolfia serpentina

ZHAO Yu, YU Rong-Min, Cornelia SCHROEDER, Ian SADLER, Matthias UNGER, SUN Xian-Feng, David W. H. RANKIN, Joachim STöCKIGT. Biotransformation of Paclitaxel (Taxol) by the Cell Suspension Cultures of Rauwolfia serpentina[J]. J Integr Plant Biol., 2004, 46(11): 1383-1386.

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Biotransformation of Paclitaxel (Taxol) by the Cell Suspension Cultures of Rauwolfia serpentina

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