J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (2): 236-241.

• Research Articles • Previous Articles     Next Articles

Identification and Molecular Mapping of a Stripe Rust Resistance Gene from a Common Wheat Line Qz180

DENG Zhi-Yong, ZHANG Xiang-Qi, WANG Xian-Ping, JING Jian-Kang, WANG Dao-Wen   

Abstract:

YrQz, a stripe rust disease resistance gene was identified in a common wheat (Triticum aestivum L.) line Qz180. This resistance was controlled by a single dominant gene, which was confirmed by genetic analysis of two F2 populations derived from the crosses using Qz180 and two susceptible parents (Mingxian 169 and WL1). Bulked segregant analysis using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers was conducted in order to map the chromosomal location of YrQz. The results indicated that YrQz was located on the long arm of wheat chromosome 2B and resided in a region flanked by two SSR loci Xgwm388 and Xgwm526. Two AFLP markers P35M48(452) and P36M61 (163) were closely linked to YrQz with the genetic distance of 3.4 cM and 4.1 cM, respectively. To our knowledge, this is the first molecularly mapped stripe rust resistance gene on wheat chromosome 2B.

Key words: wheat, stripe rust disease, disease resistance gene, AFLP, SSR

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