J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (10): 951-960.DOI: 10.1111/j.1744-7909.2009.00869.x

• Plant-environmental Interactions • Previous Articles     Next Articles

Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves

Xiao-Yue Chen1, Xiao Ding1, Sheng Xu1, Ren Wang2, Wei Xuan1, Ze-Yu Cao1, Jian Chen1, Hong-Hong Wu1, Mao-Bing Ye1 and Wen-Biao Shen1*   

  1. 1 College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique Nanjing Agricultural University and Beckman Coulter Ltd. Co., Nanjing Agricultural University, Nanjing 210095, China
    2 Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences (Mem. Sun Yat-Sen), Jiangsu Province Key Laboratory for Plant Ex-situ Conservation, Nanjing 210014, China
  • Received:2008-12-16 Accepted:2009-07-07 Published:2009-09-25
  • About author: *Author for correspondence. Tel.: +86 25 8439 8602; Fax: +86 25 8439 6542; E-mail: wbshenh@njau.edu.cn


Pretreatment of lower H2O2 doses (0.05, 0.5 and 5 mM) for 24 h was able to dose-dependently attenuate lipid peroxidation in wheat seedling leaves mediated by further oxidative damage elicited by higher dose of H2O2 (150 mM for 6 h, with 0.5 mM H2O2 being the most effective concentrations. Further results illustrated that 0.5 mM H2O2 pretreatment triggered the biphasic production of H2O2 during a 24 h period. We also noticed that only peak I (0.25 h) rather than peak II (4 h) was approximately consistent with the enhancement of heme oxygenase (HO) activity, HO-1 gene expression. Meanwhile, enhanced superoxide dismutase (SOD) activity, Mn-SOD and Cu, Zn-SOD transcripts might be a potential source of peak I of endogenous H2O2. Further results confirmed that 0.5 mM H2O2 treatment for 0.5 h was able to upregulate HO gene expression, which was detected by enzyme activity determination, semi quantitative reverse transcription-polymerase chain reaction and western blotting. Meanwhile, the application of N,N’ dimethylthiourea, a trap for endogenous H2O2, not only blocked the upregulation of HO, but also reversed the corresponding oxidation attenuation. Together, the above results suggest that endogenous H2O2 production (peak I) plays a positive role in the induction of HO by enhancing its mRNA level and protein expression, thus leading to the acclimation to oxidative stress.

Chen XY, Ding X, Xu S, Wang R, Xuan W, Cao ZY, Chen J, Wu HH, Ye MB, Shen WB (2009). Endogenous hydrogen peroxide plays a positive role in the upregulation of heme oxygenase and acclimation to oxidative stress in wheat seedling leaves. J. Integr. Plant Biol. 51(10), 951–960.

Key words: acclimation to oxidative stress, endogenous hydrogen peroxide, heme oxygenase, signal transduction, Triticum

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