TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity

doi:10.1111/jipb.13542

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (9): 2056-2070.DOI: 10.1111/jipb.13542 cstr: 32098.14.jipb.13542

• Abiotic Stress Responses • Previous Articles Next Articles

TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity

Jianhui Ma^1†, Yuke Geng^2,3†, Hong Liu^2†, Mengqi Zhang¹, Shujuan Liu², Chenyang Hao², Jian Hou², Youfu Zhang², Daijing Zhang¹, Weijun Zhang⁴, Xueyong Zhang^2* and Tian Li^2*

1. College of Life Sciences, Henan Normal University, Xinxiang 453007, China;
2. Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. College of Life and Environmental Science, Minzu University of China, Beijing 100081, China;
4. Crop Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China
^†These authors contributed equally to this work.
^*Correspondences: Tian Li (litian@caas.cn, Dr. Li is responsible for the distribution of all materials associated with this article); Xueyong Zhang (zhangxueyong@caas.cn)

Received:2022-05-09 Accepted:2023-06-12 Online:2023-06-13 Published:2023-09-01

Abstract

Abstract: Drought is a major environmental stress limiting global wheat (Triticum aestivum) production. Exploring drought tolerance genes is important for improving drought adaptation in this crop. Here, we cloned and characterized TaTIP41, a novel drought tolerance gene in wheat. TaTIP41 is a putative conserved component of target of rapamycin (TOR) signaling, and the TaTIP41 homoeologs were expressed in response to drought stress and abscisic acid (ABA). The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect. Furthermore, TaTIP41 physically interacted with TaTAP46, another conserved component of TOR signaling. Like TaTIP41, TaTAP46 positively regulated drought tolerance. Furthermore, TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities. Silencing TaPP2A-2 improved drought tolerance in wheat. Together, our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat, and their potential application in improving wheat environmental adaptability.

Key words: ABA response, drought tolerance, PP2A, TaTIP41, TaTAP46, TOR signaling, Triticum aestivum

Jianhui Ma, Yuke Geng, Hong Liu, Mengqi Zhang, Shujuan Liu, Chenyang Hao, Jian Hou, Youfu Zhang, Daijing Zhang, Weijun Zhang, Xueyong Zhang and Tian Li. TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity[J]. J Integr Plant Biol., 2023, 65(9): 2056-2070.

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TaTIP41 and TaTAP46 positively regulate drought tolerance in wheat by inhibiting PP2A activity

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