Gibberellin promotes cambium reestablishment during secondary vascular tissue regeneration after girdling in an auxin-dependent manner in Populus

doi:10.1111/jipb.13591

J Integr Plant Biol. ›› 2024, Vol. 66 ›› Issue (1): 86-102.DOI: 10.1111/jipb.13591

• Cell and Developmental Biology • Previous Articles Next Articles

Gibberellin promotes cambium reestablishment during secondary vascular tissue regeneration after girdling in an auxin-dependent manner in Populus

Yufei Zhang^†, Lingyan Wang^†, Yuexin Wu, Donghui Wang and Xin‐Qiang He^*

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
^†These authors contributed equally to this work.
^*Correspondence: Xin‐Qiang He (hexq@pku.edu.cn)

Received:2023-06-08 Accepted:2023-12-04 Online:2023-12-05 Published:2024-01-01

Abstract

Abstract: Secondary vascular tissue (SVT) development and regeneration are regulated by phytohormones. In this study, we used an in vitro SVT regeneration system to demonstrate that gibberellin (GA) treatment significantly promotes auxin-induced cambium reestablishment. Altering GA content by overexpressing or knocking down ent-kaurene synthase (KS) affected secondary growth and SVT regeneration in poplar. The poplar DELLA gene GIBBERELLIC ACID INSENSITIVE (PtoGAI) is expressed in a specific pattern during secondary growth and cambium regeneration after girdling. Overexpression of PtoGAI disrupted poplar growth and inhibited cambium regeneration, and the inhibition of cambium regeneration could be partially restored by GA application. Further analysis of the PtaDR5:GUS transgenic plants, the localization of PIN-FORMED 1 (PIN1) and the expression of auxin-related genes found that an additional GA treatment could enhance the auxin response as well as the expression of PIN1, which mediates auxin transport during SVT regeneration. Taken together, these findings suggest that GA promotes cambium regeneration by stimulating auxin signal transduction.

Key words: auxin, cambium, gibberellin, Populus, secondary vascular tissue regeneration

Yufei Zhang, Lingyan Wang, Yuexin Wu, Donghui Wang and Xin‐Qiang He. Gibberellin promotes cambium reestablishment during secondary vascular tissue regeneration after girdling in an auxin-dependent manner in Populus[J]. J Integr Plant Biol., 2024, 66(1): 86-102.

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Gibberellin promotes cambium reestablishment during secondary vascular tissue regeneration after girdling in an auxin-dependent manner in Populus

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