J Integr Plant Biol. ›› 1992, Vol. 34 ›› Issue (4): -.

• Research Articles •    

An Essential Balm Method for Preparing Rice Chromosomes

Zhuang Nan-sheng and Li Jing-du   

Abstract: An essential balm (EB) method was described for preparing mitotic chromosomes in rice (Oryza sativa L.). Briefly, root tips were pretreated with EB, fixed in 3:1 methanol-acetic acid. Chromosome specimens were made by squash and smear technique without cell wall degradation, and then stained with Giemsa. The G-bands of chromosomes could be distinctly visible with this method.

Key words: Rice, Chromosome preparation, Essential balm method

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