J Integr Plant Biol. ›› 2011, Vol. 53 ›› Issue (12): 961-970.DOI: 10.1111/j.1744-7909.2011.01082.x

• Molecular Ecology and Evolution • Previous Articles     Next Articles

RNA Editing Sites Exist in Protein-Coding Genes in the Chloroplast Genome of Cycas Taitungensis

Haiyan Chen, Likun Deng, Yuan Jiang, Ping Lu and Jianing Yu*   

  1. College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China
  • Received:2011-07-12 Accepted:2011-10-24 Published:2011-11-02
  • About author:*Corresponding author. Tel: +86 29 8531 0266; Fax: +86 29 8531 0546; E-mail: jnyu@snnu.edu.cn

Abstract:

RNA editing is a post-transcriptional process that results in modifications of ribonucleotides at specific locations. In land plants editing can occur in both mitochondria and chloroplasts and most commonly involves C-to-U changes, especially in seed plants. Using prediction and experimental determination, we investigated RNA editing in 40 protein-coding genes from the chloroplast genome of Cycas taitungensis. A total of 85 editing sites were identified in 25 transcripts. Comparison analysis of the published editotypes of these 25 transcripts in eight species showed that RNA editing events gradually disappear during plant evolution. The editing in the first and third codon position disappeared quicker than that in the second codon position. ndh genes have the highest editing frequency while serine and proline codons were more frequently edited than the codons of other amino acids. These results imply that retained RNA editing sites have imbalanced distribution in genes and most of them may function by changing protein structure or interaction. Mitochondrion protein-coding genes have three times the editing sites compared with chloroplast genes of Cycas, most likely due to slower evolution speed.

Chen H, Deng L, Jiang Y, Lu P, Yu J (2011) RNA editing sites exist in protein-coding genes in the chloroplast genome of Cycas taitungensis. J. Integr. Plant Biol. 53(12), 961–970.

Key words: chloroplasts, Cycas taitungensis, ndh genes, post-transcriptional modification, RNA editing, transcripts

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22