J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (11): 1040-1049.DOI: 10.1111/j.1744-7909.2009.00876.x

• Molecular Physiology • Previous Articles     Next Articles

Substrate Selectivity of Glycerol-3-phosphate Acyl Transferase (GPAT) in Rice

Su-Qin Zhu, Hua Zhao, Rong Zhou, Ben-Hua Ji* and Xiao-Yan Dan   

  1. School of Life Sciences, Nantong University, Nantong 226007, China
  • Received:2008-12-01 Accepted:2009-08-25 Published:2009-11-10
  • About author: *Author for correspondence Tel: +86 513 8501 5898; Fax: +86 513 8501 5908; E-mail: jibenhua@ntu.edu.cn

Abstract:

Substrate selectivity of glycerol-3-phosphate acyltransferase (EC 2. 3. 1. 15) of rice (Oryza sativa L.) was explored in a comparative study of acyltransferases from seven plant species. In vitro labeling of acyl  arrier protein (ACP) with 14C or 3H showed that acyltransferase from chill-sensitive plants, such as rice that uses either oleic (18:1) or palmitic acid (16:0) as acyl donor at comparable rates, displays lower selectivity than the enzyme from chill-resistant plants, such as spinach, which preferentially uses oleic acid (18:1) rather than palmitic acid (16:0) as an acyl donor. This may be a result of the size and character of the substrate-binding pocket of acyltransferase. Homology modeling and protein structure-based sequence alignment of acyltransferases revealed that proteins from either chill-sensitive or chill tolerant plants shared a highly conserved domain containing the proposed substrate-binding pocket. However, the aligned residues surrounding the substrate-binding pocket are highly heterogeneous and may have an influence mainly on the size of the substrate binding pockets of acyltransferases. The substrate selectivity of acyltransferase of rice can be improved by enlarging the substrate-binding pocket using molecular biological methods.
 

Zhu SQ, Zhao H, Zhou R, Ji BH, Dan XY (2009). Substrate selectivity of glycerol-3-phosphate acyl transferase in rice. J. Integr. Plant Biol. 51(11), 1040–1049.

Key words: glycerol-3-phosphate acyl transferase, homology modeling, rice, substrate selectivity.

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