J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (9): 1009-1014.

• Research Articles •     Next Articles

Effects of Exogenous Nitric Oxide on Photochemical Activity of Photosystem Ⅱ in Potato Leaf Tissue Under Non-stress Condition

YANG Jia-Ding, ZHAO Ha-Lin, ZHANG Tong-Hui, YUN Jian-Fei   


Under non-stress condition, effects of exogenous nitric oxide (NO) on chlorophyll fluorescence parameters in detached leaves and leaf discs of potato (Solanum tuberosum L.) were surveyed. Results showed that the maximal quantum efficiency (Fv/Fm) and the effective quantum efficiency (F PSⅡ) of photosystem Ⅱ (PSⅡ) were reduced by exogenous NO under illumination (150 mmol.m-2.s-1, 25 ℃). This influence was related not only to the concentration of sodium nitroprusside (SNP, a NO donor) solution, but also to the active duration of NO on leaf tissue. Results with leaf discs showed that the effects of SNP on F PSⅡ could be prevented by bovine hemoglobin (a powerful NO scavenger), while a mixture of NO2- and NO3- (the decomposition product of NO or its donor SNP) had much less influence on F PSⅡ than SNP, indicating that effects of exogenous SNP on PSⅡ photochemical activity was mainly due to NO generation. Under light (150 mmol.m-2.s-1, 25 ℃) for 4 h or longer period, the non-photochemical quenching (NPQ) in SNP-soaked leaves was statistically similar to that in H2O-soaked control, but F PSⅡ and the proportion of open reaction centers (measured as qP) were lower than control, respectively. After 25 min dark-adaptation, the maximal fluorescence (Fm) in SNP treatment (8 and 12 h illumination duration) was significantly lower than the control, while the initial fluorescence (Fo) in SNP and H2O-treated leaves had no significant difference. Therefore this indicated that under the present experimental condition, the NO-affected site might not be the PSⅡ reaction centers. On the donor side of PSⅡ, NO putatively influenced the light-harvesting capacity of leaves under light; on the acceptor side, NO-affected sites were some components of electron transport chain after QA, i.e. NO enhanced the reductive degree of reaction centers through blocking the electron transport after QA, thus reducing the photochemical activity of PSⅡ.

Key words: nitric oxide, photosystem Ⅱ (PSⅡ), quantum efficiency, potato leaf tissue, non-stress condition

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