J Integr Plant Biol. ›› 1994, Vol. 36 ›› Issue (11): -.

• Research Articles •    

Binding of Pollen Golgi Vesicles to Calf Brain Microtubule in Vitro

Liu Guo-qin, Yan Long-fei (Lung-Fei Yen), Cai G, Casino C D, Tiezzi A and Cresti M   

Abstract: The Golgi vesicles isolated from the pollen of Corylus avellana were incubated with the microtubules polymerized from highly purified calf brain tubulin. After ultracentrifugation on a layer of 1.5 mol/L sucrose, the pellets were assayed with SDS-PAGE and negative staining. The results showed that, in vitro, the pollen Golgi vesicles were able to bind onto calf brain microtubules. The microtubule-binding ability was suppressed by 10 mmol/L ATP and 0. 5 mol/L KCI, but not by 5 mmol/L AMP-PNP, suggesting that the binding may result from some ATP-related peripheral membrane proteins.

Key words: Pollen, Golgi vesicles, Microtubule-binding in vitro

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