J Integr Plant Biol. ›› 1993, Vol. 35 ›› Issue (Suppl.): -.

• Research Articles •    

Effects of Stimulation with Weak Electric Currents on in Vitro Culture of Cabbage

Wang Xiao-jia ,Wang Qiang,Song Ming and Zheng Er-xin   

Abstract: Some effects of weak electric current stimulation on in vitro shoot culture of cabbage (Brassica oleracea L. var capitata) were studied by using a new type of pusle-current-stimulator. Stainless steel was used probes and cabbage shoots in vitro culture was being tested to fined out the relationship between pulse current intensity and time of stimulation to the stimulating effects. Five current intensities as 0.43 μA, 0. 67 μA, 1.30 μA, 2.60 μA and 4.00 μA, three stimulation time as 5 days, 10 days and 20 days. Four kinds of media as MS +BA 5.0+NAA 0.1, MS+BA 3.0+NAA 0.1, MS+BA 1.0+NAA 0. 1 and MS+NAA 0. 1 (mg/L) were adopted as for different treatment. Each treatment includes two groups of different electric polarities or orientations. The number and fresh weights of shoots were measured after 20 days of culture for each treatment. The results howed that micro-current intensities within the range of 0.6—2.60μA had promoting effect on shoot differentiation and weight gain, Pulse-current could eliminate the unfavorable influence of electric polarization. Treatmint of 10 days’stimulation with 1.3 μA current gave the optimal effect. The effect was insignificant when the current was less than 0. 67μA, and was inhibitive when exceeded 2.60μA. The presence of BA in MS medium was essential for current stimulation the optimal concentration being 13.0 mg/L. As the applied current couldprovide a common polarity for ling tissues and a similar axis orientation for provide a common polarity for living tissues and a similar axis orientation for shoot growth it could promote the absorption of BA and some other important supplements with favor the differentiation and growth of the shoots in in vitroculture.

Key words: Brassica oleracea L. var capitata, In vitro culture, Pulse electrical stimulation, Development

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