J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (11): -.

• Research Articles •    

Introduction of Foreign Gene into Small Cell Groups Digested Partial-Enzymically in Rice and Regeneration of Transgenic Plants

Yang Jin-shui, Ge Kou-lin, Luo Min, Wang Yun-zhu, Wang Bei, Hu Rong-xia, Qian Min,Tan Jia-zhen (C. C. Tau), Nancy Lee and Douglas Testa   

Abstract: Suspension cultures of small cell groups (SCG; ca. 50–100 cells per group) were established from calli of Japonica rice Fang 7 and Hl24. The SCG were partially digested and transformed by plasmid pBll21 harboring the NPT-II (neomycin phosphotransferase) and GUS (betaglucuronidase) genes. Plasmid DNA was introduced into cells' by PEG, electroporation and PEG plus electroporation. NTP-II and GUS activity assay showed that the report genes were expressed in transformed cells. Transgenic plants were regeneiated possessing GUS activity due to the integration of intact foreign DNA into their genome as evidanced by hybridization. The results prove that the partially digested SCG is a potential, feasible system as receptor for gene transfer, especially for plants which are difficult for protoplast culture and plant regeneration from protoplasts.

Key words: Gene transfer, Transgenic plans of rice, GUS, NPT-11, Small cell groups digested partiial-enzymically

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