J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (10): -.

• Research Articles •    

Study on the C5-Pathway of Chlorophyll Biosynthesis in Euglena gracilis Klebs

Wen Yuan-ying   

Abstract: By feeding (l-13C)-glutamic acid to Euglena gracilis, a green alga, the 13C-NMR spectra, of chlorophyll showed that the entire carbon skeleton of glutamic acid was incorporated into chlorophyll. The existence of cspathway in chlorophyll biosynthesis was proved. In cell-free test, porphobilinogen (PBG) was formed from (5-13C)-δ-aminolaevulinic acid(ALA), but no evidence for (2-13C)-glycine incorporation was observed. It means that the Shemin pathway was effectivelessness, at least, in E. gracilis.

Key words: Euglena gracilis, Chlorophyll, C5-pathway, 13C-NMR

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