J Integr Plant Biol. ›› 1991, Vol. 33 ›› Issue (9): -.

• Research Articles •    

Oxidation of Betaine Aldehyde by Betaine Dehydrogenase from Spinach Leaves

Liang Zheng, Zhao Yuan, Li Yu-chun, Zou Yu-ping, Luo Ai-ling and Huang Ju-fu   

Abstract: Betaine content in leaves of fifteen plant species was determined. The results showed higher betaine levels in those salt-, drought-, and chilling-resistant species. Betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8 ) was isolated and partially purified from spinach leaves. Some properties of this enzyme were studied. BADH was precipitated by 60% saturation of (NH4)2SO4. Its activity was not detected in 70% saturation of (NH4)2SO4. BADH has two isoenzymes. The activity of BADH was quite stable below –80℃. It was inhibited by 0.125–1.0 mol/L NaG1 or KC1 but not by Mn2+ and Mo6+, and slightly increased by Mg2+.

Key words: Betaine aldehyde dehydrogenase, Spinach, lsoenzyme, Cation

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