J Integr Plant Biol. ›› 1992, Vol. 34 ›› Issue (11): -.

• Research Articles •    

Gliadin and Glutenin Variation in Seeds of Somaclones of Triticum aestivum

Sang Jian-li, Wang Yu-xiu and Zhu Zhi-qing   

Abstract: Gliadin and glutenin of seeds of a pollen-derived pure line “H-33” and its 9 stable somaclones of wheat (Triticum aestivurn L.) from inflorescence callus were investigated using SDSPAGE. Among the 9 somaclones 6 were identical to the “H-33” in SDS-PAGE patterns of of gliadin and glutenin. The other 3 somaclones were identical with each other, but different from “H-33”. The varied electrophoretic bands of seed protein showed that somaclonal variation had occured at gene level during tissue culture in which the somaclones were produced.

Key words: Somaclonal variation, Triticum aestivum, Gliadin, Glutenin, SDS-PAGE

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