J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (5): -.

• Research Articles •    

Transgenic Rice Plants Produced by PEG-Mediated Plasmid Uptake into Protoplasts

Li Wen-bin, Wang Ge-jiao, Stanchina E, Castiglione S, Sun Yong-ru and Sala F   

Abstract: Embryogenic cell suspension cultures were obtained from calli developed from mature rice seeds of a Japonica type Itahan cultivar, Roncarolo. Protoplasts were isolated and transformed by PEG-treated method with plasmid pHP23 carrying the NPT Ⅱ gene which encodes resistance to antibiotic G-418. Protoplast-derived colonies were selected in presence of the inhibitor. Plants were regenerated and transplanted into soil in the green house. The presence of foreign gene in the regenerated plants was verified by PCR and Southern analysis.

Key words: Rice, Protoplast, Transformation, PEG, PCR, Southern blot

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