J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (5): -.

• Research Articles •    

Observation of One Pheophytin a Molecule Not Associated with Primary Photochemistry in the Isolated Photosystem Ⅱ Reaction Center D1/D2/Cyt b559 Complex

Hou Jian-min, Peng De-chuan, Kuang Ting-yun,Tang Chong-qin and Tang Pei-song   

Abstract: Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PS Ⅱ reaction center complex. It may be considered that there are two different electron transfer branches in the PS Ⅱ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PS Ⅱ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PS Ⅱ reaction center was proposed based on our experiment.

Key words: Photosystem Ⅱ reaction center, D1/D2/Cyt b559 complex, Pheophytina, Model

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