Isolation and Characterization of Phosphatase from Thylakoid Membranes of Spinach Chloroplasts

Abstract

Abstract: A phosphatase from thylakoid membrane of spinach (Spinacia oleracea L. ) chloroplasts was isolated with the methods of extraction with n-ButanoL centrifugation at 100000 g for 30 min and chromatographic separation through DEAE-Cellulose (DE 52) column.The phosphatase catalyzed hydrolysis of phosphate monoesters (4-nitrophenyl phosphate). The optimal pH for enzyme catalysis was below 7. The peak rate of the enzyme reaction was obtained when it was incubated at 60℃ for 15 min. The phosphatase was inhibited by ATP and phosphate. The results from SDS-PAGE showed that the preparation of enzyme was composed of two proteins.

Key words: Phosphatase, 4-nitrophenyl phosphate, Thylakoid membrane, ATP

Wang Guo-qiang, Peng Jian-xin, Qian Yue-qin, Li Rong and Zhang Zheng-fu. Isolation and Characterization of Phosphatase from Thylakoid Membranes of Spinach Chloroplasts[J]. J Integr Plant Biol., 1995, 37(4): -.

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Isolation and Characterization of Phosphatase from Thylakoid Membranes of Spinach Chloroplasts

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