J Integr Plant Biol. ›› 1995, Vol. 37 ›› Issue (1): -.

• Research Articles •    

Effect of Salt Stress on Ion Channel Selective Permeability of Plasmalemma in Sorghum Roots

Pei Zhen-ming and Tang Zhang-cheng   

Abstract: 100 mmol/L, 200 mmol/L and 300 mmol/L NaC1 was used in proper order to treat three-day old seedlings of Sorghum vulgare Pets. The plasma membrane of roots was isolated and purified by aqueous biphasic partition device. The plasmalemma was incorporated into planar bilayer lipid membrane and ion channels were measured by electrical methods. Ion selective permeabilities (PK: PNa) were assayed in asymmetrical solutions containing 100 mmol/L NaC1 in Cis chamber and 100 mmol/L KC1 in Tran with chamber and were calculated from Goldman-Hodgkin-Kaltz equation. PK: PNa was 3.5 in plasmalemma of control roots and 1.5 in plasmalemma of salt stressed roots. It showed that the changes of ion selective permeability was very significant under salt stress. The importance of the change of ion selective permeability is discussed.

Key words: Bilayer lipid membrane, Ion channel, Salt stress, Plasmalemma, Sorghum

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