J Integr Plant Biol.

• Research Article •    

Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice

Kai Liu1†, Jijin Chen1†, Shang Sun2, Xu Chen2, Xinru Zhao2, Yingying Hu1, Guoxiao Qi2, Xiya Li1, Bo Xu3, Jun Miao2, Chao Xue2*, Yong Zhou1,2* and Zhiyun Gong1,2*   

  1. 1. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China;
    3. Institute of Lianyungang Agricultural Science of Xuhuai Area, Lianyungang 222006, China
    These authors contributed equally to this work.
    *Correspondences: Chao Xue (xc77seven@126.com); Yong Zhou (zhouyong@yzu.edu.cn); Zhiyun Gong (zygong@yzu.edu.cn, Dr. Gong is fully responsible for the distributions of the materials associated with this article)
  • Received:2022-09-14 Accepted:2023-02-19 Online:2023-04-19

Abstract: High salt is a major environmental factor that threatens plant growth and development. Increasing evidence indicates that histone acetylation is involved in plant responses to various abiotic stress; however, the underlying epigenetic regulatory mechanisms remain poorly understood. In this study, we revealed that the histone deacetylase OsHDA706 epigenetically regulates the expression of salt stress response genes in rice (Oryza sativa L.). OsHDA706 localizes to the nucleus and cytoplasm and OsHDA706 expression is significantly induced under salt stress. Moreover, oshda706 mutants showed a higher sensitivity to salt stress than the wild-type. In vivo and in vitro enzymatic activity assays demonstrated that OsHDA706 specifically regulates the deacetylation of lysines 5 and 8 on histone H4 (H4K5 and H4K8). By combining chromatin immunoprecipitation and mRNA sequencing, we identified the clade A protein phosphatase 2?C gene, OsPP2C49, which is involved in the salt response as a direct target of H4K5 and H4K8 acetylation. We found that the expression of OsPP2C49 is induced in the oshda706 mutant under salt stress. Furthermore, the knockout of OsPP2C49 enhances plant tolerance to salt stress, while its overexpression has the opposite effect. Taken together, our results indicate that OsHDA706, a histone H4 deacetylase, participates in the salt stress response by regulating the expression of OsPP2C49 via H4K5 and H4K8 deacetylation.

Key words: histone deacetylase, OsHDA706, OsPP2C49, rice, salt stress

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