The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis

doi:10.1111/jipb.13457

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (6): 1383-1393.DOI: 10.1111/jipb.13457

• Abiotic Stress Responses • Previous Articles Next Articles

The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis

Yechun Hong^1,2†, Yang Gao^3†, Jia Pang², Huazhong Shi⁴, Tingting Li³, Huiying Meng³, Dali Kong², Yunjuan Chen², Jian-Kang Zhu^1,2* and Zhen Wang^2,3*

1. Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
2. Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
3. School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
4. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA
^†These authors contributed equally to this work.
^*Correspondences:Jian-Kang Zhu(zhujk@sustech.edu.cn);Zhen Wang(wangzhen@ahau.edu.cn, Dr. Wang is fully responsible for the distributions of the materials associated with this article)

Received:2022-08-17 Accepted:2023-01-19 Online:2023-01-20 Published:2023-06-01

Abstract

Abstract: Salt stress adversely impacts crop production. Several spliceosome components have been implicated in regulating salt stress responses in plants, however, the underlying molecular basis is still unclear. Here we report that the spliceosomal core protein SmEb is essential to salt tolerance in Arabidopsis. Transcriptome analysis showed that SmEb modulates alternative splicing of hundreds of pre-mRNAs in plant response to salt stress. Further study revealed that SmEb is crucial in maintaining proper ratio of two RCD1 splicing variants (RCD1.1/ RCD1.2) important for salt stress response. In addition, RCD1.1 but not RCD1.2 is able to interact with the stress regulators and attenuates salt- sensitivity by decreasing salt-induced cell death in smeb-1 mutant. Together, our findings uncovered the essential role of SmEb in the regulation of alternative pre-mRNA splicing in salt stress response.

Key words: alternative splicing, RCD1, salt stress, SmEb, spliceosome

Yechun Hong, Yang Gao, Jia Pang, Huazhong Shi, Tingting Li, Huiying Meng, Dali Kong, Yunjuan Chen, Jian-Kang Zhu and Zhen Wang. The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis[J]. J Integr Plant Biol., 2023, 65(6): 1383-1393.

[1]	Jingwei Yan, Ya Liu, Jiawen Yan, Zhihui Liu, Heqiang Lou and Jiasheng Wu. The salt-activated CBF1/CBF2/CBF3-GALS1 module fine-tunes galactan-induced salt hypersensitivity in Arabidopsis [J]. J Integr Plant Biol., 2023, 65(8): 1904-1917.
[2]	Kai Liu, Jijin Chen, Shang Sun, Xu Chen, Xinru Zhao, Yingying Hu, Guoxiao Qi, Xiya Li, Bo Xu, Jun Miao, Chao Xue, Yong Zhou and Zhiyun Gong. Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice [J]. J Integr Plant Biol., 2023, 65(6): 1394-1407.
[3]	Areum Lee, Hyun Ji Park, Seung Hee Jo, Haemyeong Jung, Hyun‐Soon Kim, Hyo‐Jun Lee, Youn‐Sung Kim, Choonkyun Jung and Hye Sun Cho. The spliceophilin CYP18‐2 is mainly involved in the splicing of retained introns under heat stress in Arabidopsis [J]. J Integr Plant Biol., 2023, 65(5): 1113-1133.
[4]	Li-Xia Ye, Yan-Mei Wu, Jin-Xia Zhang, Jin-Xin Zhang, Huan Zhou, Ren-Fang Zeng, Wei-Xuan Zheng, Mei-Qi Qiu, Jing-Jing Zhou, Zong-Zhou Xie, Chun-Gen Hu and Jin-Zhi Zhang. A bZIP transcription factor (CiFD) regulates drought- and low-temperature-induced flowering by alternative splicing in citrus [J]. J Integr Plant Biol., 2023, 65(3): 674-691.
[5]	Jilin Ma, Chonghua Li, Lulu Sun, Xuechun Ma, Hui Qiao, Wenchao Zhao, Rui Yang, Susheng Song, Shaohui Wang and Huang Huang. The SlWRKY57-SlVQ21/SlVQ16 module regulates salt stress in tomato [J]. J Integr Plant Biol., 2023, 65(11): 2437-2455.
[6]	Jiaheng Yang, Xiao Qu, Tao Li, Yixiang Gao, Haonan Du, Lanjie Zheng, Manchun Ji, Paifeng Zhang, Yan Zhang, Jinxin Hu, Liangyu Liu, Zefu Lu, Zijian Yang, Huiyong Zhang, Jianping Yang, Yongqing Jiao, Xu Zheng. HY5-HDA9 orchestrates the transcription of HsfA2 to modulate salt stress response in Arabidopsis [J]. J Integr Plant Biol., 2023, 65(1): 45-63.
[7]	Xiaoliang Sun, Weiwei Qi, Yihong Yue, Huiling Ling, Gang Wang, and Rentao Song. Maize ZmVPP5 is a truncated Vacuole H⁺-PPase that confers hypersensitivity to salt stress [J]. J Integr Plant Biol., 2016, 58(6): 518-528.
[8]	Arunkumar Srinivasan, José M. Jiménez-Gómez, Fabio Fornara, Wim J. J. Soppe, and Vittoria Brambilla. Alternative splicing enhances transcriptome complexity in desiccating seeds [J]. J Integr Plant Biol., 2016, 58(12): 947-958.
[9]	Liang Yang, Qiaohong Liu, Zhibin Liu, Hao Yang, Jianmei Wang, Xufeng Li, and Yi Yang. Arabidopsis C3HC4-RING finger E3 ubiquitin ligase AtAIRP4 positively regulates stress-responsive abscisic acid signaling [J]. J Integr Plant Biol., 2016, 58(1): 67-80.
[10]	Ji-Hee Min, Jung-Sung Chung, Kyeong-Hwan Lee, and Cheol Soo Kim. The CONSTANS-like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid-dependent manner in Arabidopsis [J]. J Integr Plant Biol., 2015, 57(3): 313-324.
[11]	Pil Joon Seo. Recent advances in plant membrane-bound transcription factor research: Emphasis on intracellular movement [J]. J Integr Plant Biol., 2014, 56(4): 334-342.
[12]	Nenghui Ye, Guozhen Yang, Yan Chen, Chan Zhang, Jianhua Zhang, and Xinxiang Peng. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice [J]. J Integr Plant Biol., 2014, 56(2): 170-180.
[13]	Derui Liu, and Xiuling Cai. OsRRMh, a Spen-like Gene, Plays an Important Role during the Vegetative to Reproductive Transition in Rice [J]. J Integr Plant Biol., 2013, 55(9): 876-887.
[14]	Huijuan Zhang, Jing Zhai, Jibo Mo, Dayong Li and Fengming Song. Overexpression of Rice Sphingosine-1-Phoshpate Lyase Gene OsSPL1 in Transgenic Tobacco Reduces Salt and Oxidative Stress Tolerance [J]. J Integr Plant Biol., 2012, 54(9): 652-662.
[15]	Fan Lin, Yun Zhang and Ming-Yi Jiang. Alternative Splicing and Differential Expression of Two Transcripts of Nicotine Adenine Dinucleotide Phosphate Oxidase B Gene from Zea mays, [J]. J Integr Plant Biol., 2009, 51(3): 287-298.

The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis

HTML

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments