Identification of Myosin on the Surface of Wheat Mitochondria

Abstract

Abstract: It was demonstrated that myosin was associated with the surface of mitochondria in wheat ( Triticum aestivum L. ). Assays of polyacrylamide gel electrophoresis and Western blotting have shown that a polypeptide with molecular weight of 210 kD could be recognized by a polyclonal antibody against hmnan muscle myosin. It was found that the ATPase activity of mitochondrial suspension could be stimulated by F-actin isolated from chicken muscle, which indicated that there was myosin on the surface of wheat mitochondria. This result was confirmed by electron microscopic observation: mitochondria treated with N-ethylmaleimide (NEM) could be wrapped by the F-actin.

Key words: Mitochondria in wheat, Myosin, F-actin, ATPase, Localization

ZHAO He-Ping, LIU Ai-Xiao, REN Dong-Tao, LIU Guo-Qin and YAN Long-Fei (YEN Lung Fei). Identification of Myosin on the Surface of Wheat Mitochondria[J]. J Integr Plant Biol., 1999, 41(12): -.

[1]	Da Been Kim, Changhee Na, Inhwan Hwang and Dong Wook Lee. Understanding protein translocation across chloroplast membranes: Translocons and motor proteins [J]. J Integr Plant Biol., 2023, 65(2): 408-416.
[2]	Qi-Na Huang, Yong-Feng Shi, Xiao-Bo Zhang, Li-Xin Song, Bao-Hua Feng, Hui-Mei Wang, Xia Xu, Xiao-Hong Li, Dan Guo, and Jian-Li Wu. Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice [J]. J Integr Plant Biol., 2016, 58(1): 12-28.
[3]	Limor Buchnik, Mohamad Abu-Abied and Einat Sadot. Role of plant myosins in motile organelles: Is a direct interaction required? [J]. J Integr Plant Biol., 2015, 57(1): 23-30.
[4]	Chris Hawes, Petra Kiviniemi and Verena Kriechbaumer. The endoplasmic reticulum: A dynamic and well-connected organelle [J]. J Integr Plant Biol., 2015, 57(1): 50-62.
[5]	Peter K. Hepler, and Lawrence J. Winship. The pollen tube clear zone: Clues to the mechanism of polarized growth [J]. J Integr Plant Biol., 2015, 57(1): 79-92.
[6]	Julianna K. Vick and Andreas Nebenführ. Putting On The Breaks: Regulating Organelle Movements in Plant Cells† [J]. J Integr Plant Biol., 2012, 54(11): 868-874.
[7]	Liming Zhou, Ying Fu, and Zhenbiao Yang. A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes [J]. J Integr Plant Biol., 2009, 51(8): 751-761.
[8]	Hong-Mei Teng, Min-Feng Fang, Xia Cai, and Zheng-Hai Hu. Localization and Dynamic Change of Saponin in Vegetative Organs of Polygala tenuifolia [J]. J Integr Plant Biol., 2009, 51(6): 529-536.
[9]	Shi-Biao Liu, Xu-Chu Wang, Min-Jing Shi, Yue-Yi Chen, Zheng-Hai Hu, and Wei-Min Tian. Vegetative Storage Protein with Trypsin Inhibitor Activity Occurs in Sapindus mukorassi, a Sapindaceae Deciduous Tree [J]. J Integr Plant Biol., 2009, 51(4): 352-359.
[10]	Bao-Quan Liu, Lifeng Jin, Lei Zhu, Jiejie Li, Shuli Huang and Ming Yuan. Phosphorylation of Microtubule-associated Protein SB401 from Solanum Berthaultii Regulates Its Effect on Microtubules [J]. J Integr Plant Biol., 2009, 51(3): 235-242.
[11]	Liqin Wei and Yan Li. Distribution of an Ankyrin-repeat Protein on the Endoplasmic Reticulum in Arabidopsis [J]. J Integr Plant Biol., 2009, 51(2): 140-146.
[12]	Lingling Tan, Zhenghai Hu, Xia Cai and Xilu Ni. Localization and Dynamic Change of Saikosaponin in Root of Bupleurum chinense DC. [J]. J Integr Plant Biol., 2008, 50(8): 951-957.
[13]	Yan Zhu, Ziyu Li, Ben Xu, Hongda Li, Lingjian Wang, Aiwu Dong, Hai Huang. Subcellular Localizations of AS1 and AS2 Suggest Their Common and Distinct Roles in Plant Development [J]. J Integr Plant Biol., 2008, 50(7): 897-905.
[14]	Ling Shao, Zhan Shu, Shu-Lan Sun, Chang-Lian Peng , Xiao-Jing Wang and Zhi-Fang Lin. Antioxidation of Anthocyanins in Photosynthesis Under High Temperature Stress [J]. J Integr Plant Biol., 2007, 49(9): 1341-1351.
[15]	Chun-Li Li, Zhi-Ling Chen and Ming Yuan. Actomyosin is Involved in the Organization of the Microtubule Preprophase Band in Arabidopsis Suspension Cultured Cells [J]. J Integr Plant Biol., 2006, 48(1): 53-61.

Identification of Myosin on the Surface of Wheat Mitochondria

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments