Abstract: It was demonstrated that myosin was associated with the surface of mitochondria in wheat ( Triticum aestivum L. ). Assays of polyacrylamide gel electrophoresis and Western blotting have shown that a polypeptide with molecular weight of 210 kD could be recognized by a polyclonal antibody against hmnan muscle myosin. It was found that the ATPase activity of mitochondrial suspension could be stimulated by F-actin isolated from chicken muscle, which indicated that there was myosin on the surface of wheat mitochondria. This result was confirmed by electron microscopic observation: mitochondria treated with N-ethylmaleimide (NEM) could be wrapped by the F-actin.

Key words: Mitochondria in wheat, Myosin, F-actin, ATPase, Localization