J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (9): -.

• Research Articles •    

Purification and Identification of Antifreeze Proteins in Ammopiptanthus mongolicus

JIANG Yong, WEI Ling-Bo, FEI Yun-Biao, SHU Nian-Hong and GAO Su-Qin   

Abstract: The conventional protein chromatography technique was adopted to purify the antifreeze proteins (AFPs) from the leaves of Ammopiptanthus mongolicus ( Maxim. ) Cheng f. Two bands on native PAGE gel showed thermal hysteresis activity, one was band Bi, whose thermal hysteresis was 0.46 cE at 8 g/L, which showed two bands (67 kD, 21 kD) on SDS-PAGE gel; the other was B3, whose thermal hysteresis was 0.45 cE at 10 g/L, and it contained only a single protein (39.8 kD). Both B1 and B3 are not glycoproteins, because neither do they interact with Shift-reagent, nor show ultraviolet characteristics of a typical glycoprotein.

Key words: Ammopiptanthus mongolicus, Antifreeze proteins, Chromatography, Electrophoresis elution

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