J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (7): -.

• Research Articles •    

Detection of the Rye Chromatin in Multispikelet Wheat Germplasm 10-A Background Using Fluorescence in situ Hybridization (FISH) and RFLP Markers

WEI Yu-Ming, ZHENG You-Liang, ZHOU Rong-Hua and JIA Ji-Zeng   

Abstract: The multispikelet genes of rye ( Secale cereale L. ) in common wheat ( Triticum aestivum L. ) line 10-A were detected by APAGE, fluorescence in situ hybridization (FISH) and RFLP markers. APAGE analysis indicated that the 10-A possesed the gliadin marker GldlB3 of 1RS. Using fluorescence labeled total genomic DNA of rye as probes and common wheat genomie DNA for blocking, in sim hybridization showed that 1RS of rye was transferred to multispikelet wheat line 10-A. The restriction fragments located on the short ann of chromosome lB were missing and the restriction fragments of IRS were present when the probes, which have been identified on the short ann of the homologous group 1, were used in RFLP analysis. These results suggested that the multispikelet wheat line 10-A carried the 1RS/1BL wheat-rye translocation chromosome. The utilization of 10-A in wheat improvement was discussed.

Key words: Wheat, Rye, Multispikelet wheat, 1RS/1BL translocation, Ghadin, Fluorescence in situ hybridization, RFLP

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