J Integr Plant Biol. ›› 1985, Vol. 27 ›› Issue (2): -.

• Research Articles •    

Purification and Characterization of Low Molecular Weight RNA of Chloroplasts

Cheng Zhen-qi, Zhang Hung Gu Li-ya, Zhao Kun and Cao Wei-guo   

Abstract: Purified intact chloroplasts were obtained from plant leaves by homo- genizing at low speed and filtration. The chloroplast RNA were extracted from the chloroplasts with a mixture of extracting buffer and phenol. The chloroplasts of Oenan the stolonifera DC. contain not only 5S RNA and 4S RNA but also 4.5S RNA among low molecular weight RNA. However there are only 5S RNA and 4S RNA in chloroplasts of Metasequoia glyptostroides and Ginkgo biloba leaves.

Key words: Chloroplast, Chloroplast RNA, Low molecular RNA, Purification

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