Purification and Characterization of Total mRNA from  Apium graveoleus L.Leaves

Abstract

Abstract: The common procedure for purification of total messenger RNA (mRNA) from plant leaves, Apium graveoleus L. as material, has been described. The activities of protein synthesis and reverse transcription of the mRNA were determined. The leaves of Apium graveoleus L. different stage of growth contain variable content of the RNA (total RNA and mRNA). The length and distribution of the poly(A) at 3′-end of the mRNA were estimated. The maxium length of poly(A) were determined as 165 ± 5 A-residues.

Key words: messenger RNA, Complementary DNA, translation, transcription, Purification

Cheng Zhen-qi(Cheng Chen-chi), Lü Jun-xuan, Zhang Lan-fang, Wei Xi-ping and Zhao Kun. Purification and Characterization of Total mRNA from Apium graveoleus L.Leaves[J]. J Integr Plant Biol., 1984, 26(1): -.

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Purification and Characterization of Total mRNA from Apium graveoleus L.Leaves

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