Abstract:

RAPD assessment of genetic variations in 33 varieties of banana ( Musa nana Lour.) was carried out. Eighteen primers were screened from 249 10 bp arbitrary primers, and a total of 192 DNA bands were amplified, among which 183 (95.31%) were polymorphic. The average number of DNA bands amplified by each primer was 10.67, and the average genetic distance among 33 varietieswas 0.3412. Based on UPGMA cluster analysis of 192 DNA bands amplified by 18 primers a DNA molecular dendrogram was established for 33 varietiesof banana in China, which divided the 33 varieties into four groups: group A,B,C and D. Group A included 20 varieties, group B included 5 varieties, group C included 2 varieties and group D included 6 varieties. Group A could be divided into 3 sub-groups: A1, A2 and A3. The molecular foundation of genetic diversity of banana was also explored.

香蕉33个品种的RAPD研究
杜道林^1,2  苏杰¹ 周鹏² 郑学勤² 黄秉智³ 李丰年³

（1.  海南师范学院生物系，海口571185；2. 中国热带农业科学院热带作物生物技术国家重点实验室，海口571101；3.广东省农业科学院果树研究所，广州%!510640）

摘要：利用RAPD技术对香蕉(Musa nana Lour.) 33个品种的遗传变异进行了研究.从249个随机引物中筛选出18个有效引物,用它们共扩增出192条DNA带,其中183条为多态性带,占95.31%,平均每个引物扩增的DNA带数为10.67条.利用18个有效引物扩增的192条DNA带对香蕉33个品种间的亲缘关系进行UPGMA聚类分析,计算出33个品种间的平均遗传距离为0.341*!2.在此基础上建立了香蕉33个品种的DNA 分子系统树状图.该系统将香蕉33个品种划归A、B、C和D 4个群,其中A群20个品种,B群5个品种,C群2个品种,D群6个品种;A群又可以分为3个亚群.对香蕉遗传多样性分子基础进行了探讨.

关键词： 香蕉；品种；RAPD；遗传多样性

Key words: banana, variety, RAPD, genetic diversity