J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (10): -.

• Research Articles •    

Promoter Function of a Rice Repetitive DNA Sequence RRD3 in Transgenic Plants

WANG Jin-Fa, LI Yi-Kun, HE Hai-Qiong, CHEN Zhong-Jian, LIU Liang-Shi   

Abstract: A 820 bp rice (Oryza sativa L.) repetitive DNA sequence, the RRD3, was cloned by annealing kinetics. From the sequence analysis, there are several conserved promoter motifs in the sequence such as TATA-box, CAAT-box, etc. In order to detect the promoter function of RRD3, RRD3 was inserted into Ti plasmid pBI121 to replace the CaMV 35S promoter DNA fragment. Both transgenic tobacco (Nicotiana tabacum L.) G28 and rice callus showed the β-glucuronidase (GUS) activity by histochemical assays, the GUS activities of the transgenic tobacco were primarily localized at or around the vascular tissue in leaf and stem. These results indicated that RRD3 can exercise promoter function. The core sequence of promoter of RRD3 will be located.

Key words: rice, repetitive DNA sequence, promoter, GUS histochemical assays

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