J Integr Plant Biol. ›› 1999, Vol. 41 ›› Issue (3): -.

• Research Articles •    

Characterization and Structural Analysis of a SOD-like Material from Rose Fruit

GU Xue-Song, LIN Yong-Qi and ZHU Yu-Xian   

Abstract: It is known that SOD activity in rose ( Rosa rugosa var. chamissoniana) fruit is very high. By organic solvent fraction and dialysis, the authors found that the substance of scavenging 02- is mainly a material with low molecular weight (termed as SOD-like material). Ultraviolet spectrophotometry, CD spectra, amino acids analysis and iron determination of the purified peptide indicated that it has approximately 70 amino acids and one atom of iron per molecule. The Cys content of SOD-like material is higher than that of SOD. In the ultraviolet region, a peak at 280 nm was observed. CD spectra showed a characteristic peak of random coil. The N-terminal group of this molecule, as determined by Pansyl Chloride method, was not enzymatically cleaveable. This SOD-like material also has very high temperature and acidic stability.

Key words: SOD-like material, Rose, Fruit, Purification and isolation

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