J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (2): -.

• Research Articles •    

Microdissection and PCR Amplification of Rye Chromosome

SONG Wen-Qin, LI Xiu-Lan, XU Wen-Sheng and CHEN Rui-Yang   

Abstract: The 18 ( 14A + 4B) chromosomes of rye ( Secale cereale L. ) were isolated by microdissection technique and the chromosomal DNA was amplified using the artificial synthetic single unique oligonucleotide-primed polymerase chain reaction (SUP-PCR). Southern hybridization demonstrated that the SUP-PCR products have homology with rye genome DNA.

Key words: Chromosome of rye, Microdissection, PCR amplification

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