J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (8): -.

• Research Articles •    

Standardization of Electrophoregrams for Classification and Identification of Wheat Cultivars

Sun Zhi-liang, Jiang Jia-hui, Liang Yong-ping, Sun Mei-juan, Tan Ye-jie and Peng Shou-hua   

Abstract: Electrophoregrams of four wheat ( Triticum aestivum L. ) cultivars were evaluated for band deviations and control of methods using 9% acrylamide gels in a glycine acetic acid buffer at pH 3.10. The regular patterns of band deviation changed with its migration distance which revealed that the farther the band moved, the greater its standard deviation and range. Comparing with band distance of 2.05 cm, the band at 9.25 cm had a three fold increased standard deviation of 0.033 2 and the range increase from 1.5 mm to 4.5 mm. No biotypes of electrophoregrams was observed in "Yannong 15" ,"Jinan 13" and "Laizhou 953". The results demonstrated that "Laizhou 953" and "Jinan 13" were better reference cuhivars than "Marquis". Four marker bands 2.05 cm,3.15 cm, 6.05 cm and 9.25 cm were chosen based on the assessment of the electrophoregrams. In accordance with the standard averages of these markers and their actural migrating distances in a nm a calibration coefficient and calibrated migrating distance could be computed for each band on an elec- trophoregram. Thus a two third reduction of the standard deviation could be expected.

Key words: Electrophoregram, Deviation, Reference cultivars, Marker band, Triticum aestivum

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