J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (8): -.

• Research Articles •    

Microdissection and Recovery of 1R Chromosome in Rye ( Secale cereale )

Cui Li-hua, Hu Zan-min, Wang Lan-lan, Li Liang-cai and Chen Zheng-hua   

Abstract: The technique of mieromanipulaion has been used to establish a system of single chromosome mierodissection. According to the standard karyotype of rye ( Secale cereale L. ), 1R chromosome carrying disease-resistant gene has been identified, microdissected and transferred into Ep-pendorf tube using mieromanipulator. The results showed that IR chromosome in cells pretreated with α-bromonaphthalene could be identified quickly and the efficiency of microdissection was greatly improved when the technique of wall degradation and hypotonic treatment was applied.

Key words: Secale cereale, IR chromosome, Wall degradation and hypotonic treatment, Microdissection

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