Oilseed Rape Transformation and the Establishment of a BromoxynilResistant Transgenic Oilseed Rape

Abstract

Abstract: Using hypocotyls and cotyledons as transformed materials, an efficient transformation system of oilseed rape (Brassica napus L.) was established. Bxn gene (bromoxynil-resistant gene)was introduced into these plants, and bromoxynil-resistant transgenic oilseed rape was obtained. Themolecular monitoring experiments showed that these transgenic plants contained bxn gene. The herbicide experiments showed that these transgenic plants had resistance to 10-3 moL/Lbromoxynil.

Key words: Brassica napus, Transformation, bxn gene, Bromoxynil-resistant transgenic oilseed rape

Zhong Rong,Zhu Feng,Liu Yu-le,Li Sheng-guo,Kang Liang-yi and Luo Peng. Oilseed Rape Transformation and the Establishment of a BromoxynilResistant Transgenic Oilseed Rape[J]. J Integr Plant Biol., 1997, 39(1): -.

[1]	Hee-Jeong Jeong, and Ki-Hong Jung. Rice tissue-specific promoters and condition-dependent promoters for effective translational application [J]. J Integr Plant Biol., 2015, 57(11): 913-924.
[2]	Sarah Hatzig, L. Irina Zaharia, Suzanne Abrams, Marie Hohmann, Laurie Legoahec, Alain Bouchereau, Nathalie Nesi, and Rod J. Snowdon. Early osmotic adjustment responses in drought-resistant and drought-sensitive oilseed rape [J]. J Integr Plant Biol., 2014, 56(8): 797-809.
[3]	Xue-Long Wu, Zhi-Hong Liu, Zhang-Hua Hu and Rui-Zhi Huang. BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed [J]. J Integr Plant Biol., 2014, 56(6): 582-593.
[4]	Wang Li, Bing Wang, Man Wang, Min Chen, Jing-Ming Yin, Ghullam Murtaza Kaleri, Rui-Jie Zhang, Tie-Niu Zuo, Xiong You and Qing Yang. Cloning and characterization of a potato StAN11 gene involved in anthocyanin biosynthesis regulation [J]. J Integr Plant Biol., 2014, 56(4): 364-372.
[5]	Wenxiang Zhang, Shuli Fan, Chaoyou Pang, Hengling Wei, Jianhui Ma, Meizhen Song, and Shuxun Yu. Molecular Cloning and Function Analysis of Two SQUAMOSA-Like MADS-Box Genes From Gossypium hirsutum L. [J]. J Integr Plant Biol., 2013, 55(7): 597-607.
[6]	Jinjin Jiang, Yanlin Shao, Aimin Li, Chunliang Lu, Yongtai Zhang, and Youping Wang. Phenolic Composition Analysis and Gene Expression in Developing Seeds of Yellow- and Black- seeded Brassica napus [J]. J Integr Plant Biol., 2013, 55(6): 537-551.
[7]	Liguo Jia, Qiuyu Wu, Nenghui Ye, Rui Liu, Lu Shi, Weifeng Xu, Hui Zhi, A. N. M. Rubaiyath Bin Rahman, Yiji Xia, and Jianhua Zhang. Proanthocyanidins Inhibit Seed Germination by Maintaining High Level of Abscisic Acid in Arabidopsis thaliana [J]. J Integr Plant Biol., 2012, 54(9): 663-673.
[8]	Stefan Bieker, Lena Riester, Mark Stahl, Jürgen Franzaring, and Ulrike Zentgraf. Senescence-specific Alteration of Hydrogen Peroxide Levels in Arabidopsis thaliana and Oilseed Rape Spring Variety Brassica napus L. cv. Mozart [J]. J Integr Plant Biol., 2012, 54(8): 540-554.
[9]	Martin A. J. Parry, and Malcolm J. Hawkesford. An Integrated Approach to Crop Genetic Improvement [J]. J Integr Plant Biol., 2012, 54(4): 250-259.
[10]	Jia Liu, Qijie Zheng, Qiuxiang Ma, Kranthi Kumar Gadidasu, and Peng Zhang. Cassava Genetic Transformation and its Application in Breeding [J]. J Integr Plant Biol., 2011, 53(7): 552-569.
[11]	Reza Maali Amiri, Natalia O. Yur'eva, Khristina R. Shimshilashvili, Irina V. Goldenkova-Pavlova, Vasiliy P. Pchelkin, Elmira I. Kuznitsova, , Vladimir D. Tsydendambaev, Tamara I. Trunova, Dmitry A. Los, Gholamreza Salehi Jouzani, and Alexander M. Nosov, . Expression of Acyl-lipid Δ12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato [J]. J Integr Plant Biol., 2010, 52(3): 289-297.
[12]	Yu-Qing He and Yan Wu. Oil Body Biogenesis during Brassica napus Embryogenesis [J]. J Integr Plant Biol., 2009, 51(8): 792-799.
[13]	Hai-Hong Shang, Chuan-Liang Liu, Chao-Jun Zhang, Feng-Lian Li, Wei-Dong Hong and Fu-Guang Li. Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton [J]. J Integr Plant Biol., 2009, 51(5): 456-465.
[14]	Lin Cheng, Yijing Zou, Shuli Ding, Jiajing Zhang, Xiaolin Yu, Jiashu Cao and Gang Lu. Polyamine Accumulation in Transgenic Tomato Enhances the Tolerance to High Temperature Stress [J]. J Integr Plant Biol., 2009, 51(5): 489-499.
[15]	Zhong Zhao, Yan Zhu, Mathieu Erhardt, Ying Ruan, and Wen-Hui Shen. A Non-canonical Transferred DNA Insertion at the BRI1 Locus in Arabidopsis thaliana [J]. J Integr Plant Biol., 2009, 51(4): 367-373.

Oilseed Rape Transformation and the Establishment of a BromoxynilResistant Transgenic Oilseed Rape

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments