J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (5): -.

• Research Articles •    

Mass Isolation and Purification of Generative Cells from Pollen Grains

Zhou Chang and Wu Xin-li   

Abstract: A technique named “two-step osmotic shock” was developed for the isolation of large number of generative cells from pollen grains in Vicia faba and Zephyranthes candida. The procedure included: 1. Pollen hydration: mature pollen grains were incubated in adequate sucrose solution for 30-50 min. 2. Osmotic shock, when the pollen grains swelled and showed the tendency of germination, a sudden osmotic shock by adding equal volume of water made them bursting. The contents carrying generative cells spurted from the germ pores. By subsequent filtration and centrifugation, purified generative cell population was obtained. FDA test proved that the isolated and purified generative cells were viable.

Key words: Pollen generative cell, Isolation and purification, Vicia faba, Zephyranthes Candida, Z. grandiflora

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q