J Integr Plant Biol. ›› 1986, Vol. 28 ›› Issue (4): -.

• Research Articles •    

Biochemical Characteristics of Pollen Wall Glycoproteins Separated on Ricin-Sepharose 4B in Cucurbita pepo

Li Xiong-biao, Yang Zhong-han and Cao Zong-xun (Tsao, T. H.)   

Abstract: By affinity chromatography on ricin-Septarose 4B. pollen wall glycoproteins of Cucurbita pepo were isolated from pollen wall proteins which were prepared by extracting the pollen with isotonic Tris-HC1 buffer. Gas chromatographic analyses showed that the carbohydrate fraction of the pollen wall glycoproteins contained 0.7% rhamnose, 8.1% fucose, 5.1% arabinose, 5.1% xylose, 36.5% galactose, 36.2% glucose, and two unknown components. Isoelectric focusing PAGE showed that the pollen wall glycoproteins contained 6 components, with their respective pI at 3.8, 4.8, 5.4, 6.2, 6.7 and 7.6, and that the relative content of each of them was 40.6%, 15.6% 8.3%, 14.4%, 7,9%, and 13.2%, respectively. The amino acid composition of the 6 glycoproteins was determined with amino acid analyzer. Serine, glutamic acid and glycine were some of the predominent amino acids. These three, amino acids constitute more than 49% of the total amino acids.

Key words: Cucurbita pepo, Pollen wall, Glycoprotein, Affinity chromatography

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