ORF355 confers enhanced salinity stress adaptability to S-type cytoplasmic male sterility maize by modulating the mitochondrial metabolic homeostasis

doi:10.1111/jipb.13382

J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (3): 656-673.DOI: 10.1111/jipb.13382 cstr: 32098.14.jipb.13382

• Abiotic Stress Responses • Previous Articles Next Articles

ORF355 confers enhanced salinity stress adaptability to S-type cytoplasmic male sterility maize by modulating the mitochondrial metabolic homeostasis

Senlin Xiao^1†, Wei Song^1†, Jinfeng Xing¹, Aiguo Su¹, Yanxin Zhao¹, Chunhui Li¹, Zi Shi¹, Zhiyong Li¹, Shuai Wang¹, Ruyang Zhang¹, Yuanrong Pei², Huabang Chen^2* and Jiuran Zhao^1*

1. Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
2. State Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China
^†These authors contributed to this work equally.
^*Correspondences: Huabang Chen (hbchen@genetics.ac.cn); Jiuran Zhao (maizezhao@126.com, Dr. Zhao is fully responsible for the distribution of all materials associated with this article)

Received:2022-07-26 Accepted:2022-10-09 Online:2022-10-12 Published:2023-03-01

Abstract

Abstract: Moderate stimuli in mitochondria improve wide-ranging stress adaptability in animals, but whether mitochondria play similar roles in plants is largely unknown. Here, we report the enhanced stress adaptability of S-type cytoplasmic male sterility (CMS-S) maize and its association with mild expression of sterilizing gene ORF355. A CMS-S maize line exhibited superior growth potential and higher yield than those of the near-isogenic N-type line in saline fields. Moderate expression of ORF355 induced the accumulation of reactive oxygen species and activated the cellular antioxidative defense system. This adaptive response was mediated by elevation of the nicotinamide adenine dinucleotide concentration and associated metabolic homeostasis. Metabolome analysis revealed broad metabolic changes in CMS-S lines, even in the absence of salinity stress. Metabolic products associated with amino acid metabolism and galactose metabolism were substantially changed, which underpinned the alteration of the antioxidative defense system in CMS-S plants. The results reveal the ORF355-mediated superior stress adaptability in CMS-S maize and might provide an important route to developing salt-tolerant maize varieties.

Key words: cytoplasmic male sterility, metabolic homeostasis, mitochondria, salinity stress adaptability, Zea mays L.

Senlin Xiao, Wei Song, Jinfeng Xing, Aiguo Su, Yanxin Zhao, Chunhui Li, Zi Shi, Zhiyong Li, Shuai Wang, Ruyang Zhang, Yuanrong Pei, Huabang Chen and Jiuran Zhao. ORF355 confers enhanced salinity stress adaptability to S-type cytoplasmic male sterility maize by modulating the mitochondrial metabolic homeostasis[J]. J Integr Plant Biol., 2023, 65(3): 656-673.

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ORF355 confers enhanced salinity stress adaptability to S-type cytoplasmic male sterility maize by modulating the mitochondrial metabolic homeostasis

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