Please wait a minute...
J Integr Plant Biol, 2005, 47 (1): 92-99, Research Article
OsHT, a Rice Gene Encoding for a Plasma-Membrane Localized Histidine Transporter
Di LIU, Wei GONG, Yong BAI, Jing-Chu LUO and Yu-Xian ZHU
doi: 10.1111/j.1744-7909.2005.00016.x
Abstract
Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OsHT (histidine transporter of Oryza sativa L.) by screening the rice cDNA library. The cDNA is 1.3 kb in length and the open reading frame encodes for a 441 amino acid protein with a calculated molecular mass of 49 kDa. Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters. Computational analysis indicated that OsHT is an integral membrane protein with 11 putative transmembrane helices. This was confirmed by the transient expression assay because the OsHT-GFP fusion protein was, indeed, localized mainly in the plasma membrane of onion epidermal cells. Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae, suggesting that OsHT is a gene that encodes for a histidine transporter from rice. This is the first time that an LHT-type amino acid transporter gene has been cloned from higher plants other than Arabidopsis.
--------------------------------------------------------------------------------------------------------------------
   Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Di LIU
   Articles by Wei GONG
   Articles by Yong BAI
   Articles by Jing-Chu LUO
   Articles by Yu-Xian ZHU
PubMed
   Articles by Di LIU
   Articles by Wei GONG
   Articles by Yong BAI
   Articles by Jing-Chu LUO
   Articles by Yu-Xian ZHU
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22